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. 2011 Mar 16;286(19):17079–17090. doi: 10.1074/jbc.M110.192856

FIGURE 6.

FIGURE 6.

Rb and hVam6p interaction domains are discrete sites on LT. A, the LT.LXCXK mutant fails to bind Rb. U2OS cell lysates co-expressing HA-Rb with empty vector, LT, or Rb-binding mutant LT.LXCXK were immunoprecipitated for LT by anti-V5 antibody and immunoblotted for HA-Rb. B, LT.LXCXK mutant retains hVam6p interaction. U2OS cell lysates co-expressing HA-hVam6p with empty vector, LT, or Rb-binding mutant LT.LXCXK were immunoprecipitated for LT by anti-V5 antibody and immunoblotted for HA-hVam6p. C, LT.W209A mutant has no effect on Rb binding. U2OS cell lysates co-transfected with empty vector, LT, or LT.W209A were immunoprecipitated for LT by anti-V5 antibody and immunoblotted for HA-Rb. D, hVam6p does not associate with Rb in the presence or absence of LT. Left panel, 5% lysate input for LT, hVam6p, and Rb. Right panel, immunoprecipitation (IP) and immunoblotting (IB) for Rb.