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. 2011 Mar 23;286(19):17103–17111. doi: 10.1074/jbc.M110.208082

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© 2011 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 3. — Differential activity of DALLY and DLP in BMP trans signaling. Trans signaling was assayed using different combinations of the two ligands (DPP and GBB) and the two glypicans (DALLY and DLP), by the immunoblot-based trans signaling assay (A) and single cell assay (B and C). Quantification for the single cell assay using DPP and GBB are shown in B and C, respectively. p values are represented as in Fig. 1E. D, anti-pMAD staining of wing discs overexpressing dally and dlp in the dorsal compartment by ap-Gal4. D and V indicate the dorsal (dashed line) and ventral compartments, respectively. Expression of dally but not dlp expands the DPP activity gradient (arrows).