FIGURE 4.

Biphasic activity of DALLY in DPP signaling. A–C, anti-pMAD staining of wild-type (A) and dally mutant (B) wing discs. Intensity plots of pMAD signals along the anterior-posterior axis in A (wild-type; dotted line) and B (dally; solid line) are shown in C. D, effects of DALLY on DPP signaling under different ligand concentrations. The sending cells were transfected with the indicated amount of dpp cDNA (ng) with or without dally. After co-culture with the receiving cells expressing Mad-FLAG, levels of pMAD and MAD-FLAG were analyzed by immunoblotting using anti-pMAD and anti-FLAG antibodies, respectively. DPP-HA in the conditioned medium was measured using anti-HA antibody.