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. 2011 Mar 14;286(19):17193–17204. doi: 10.1074/jbc.M110.205146

FIGURE 4.

FIGURE 4.

Identification of genes dysregulated in Jarid2 mutant mice. A–C, qRT-PCR was performed on wild type and Jarid2 KO hearts at E10.5 (A), E12.5 (B), and E15.5 (C) for Notch1, Nrg1, Nrg4, Jagged1, ErbB2, ErbB4, Notch4, Hey1, Hey2, and Delta4. For all qRT-PCR, at least three hearts for each stage were used, and qRT-PCR was performed in duplicate. An asterisk indicates a significant difference when p values are less than 0.05 from a Student's paired t test, and error bars represent the standard error of the mean. D–K, section in situ hybridization was performed using DIG-labeled Notch1 riboprobes on wild type and Jarid2 KO mice at E10.5 (D–G) and E12.5 (H–K). Jarid2 KO mice exhibit higher levels of Notch1, and arrows indicate deeper invagination of endocardium into compact layer in Jarid2 KO heart (K) as compared with wild type (J). rv, right ventricle; lv, left ventricle. L–O, section in situ hybridization was performed using DIG-labeled Nrg1 riboprobes on wild type (L and N) and Jarid2 KO (M and O) at E13.5. Jarid2 KO mice exhibit higher levels of Nrg1. rv, right ventricle; lv, left ventricle. P–S, section in situ hybridization was performed on E13 control (P and R) and Jarid2en (Q and S) mice using a DIG-labeled Notch1 riboprobe. Jarid2en mice exhibit higher levels of Notch1 than a littermate control. The scale bars in D, H, L, and P represent 200 μm.