FIGURE 6.
Jarid2 occupies a specific site at the Notch1 locus. A, a VISTA alignment was performed on the Notch1 locus for mouse, monkey, human, and rat to determine conserved regions. In the bottom panel, gray bars indicate regions with greater than 75% sequence conservation, whereas black bars indicate highly conserved exon regions. Arrowheads indicate primer sites in conserved regions. B, Jarid2 occupies a specific region at +1150 bp of the Notch1 locus. qChIP was performed four times on 10 pooled E17 wild type mouse hearts using either preimmune (Pre-IM, gray bar) or a Jarid2 specific antibody (Jarid2 antibody, black bar). IP, immunoprecipitation. C, Jarid2 does not accumulate at the +1150-bp region of the Notch1 locus when Jarid2 is deleted in the endocardium. qChIP was performed three times on three pooled E17 control (gray bar) or Jarid2en (black bar) mouse hearts using a Jarid2 antibody. D, luciferase reporter gene assay. pGL3, pGL3-Notch1 −500 to +350 (N+350), and pGL3-Notch1 −500 to +1750 (N+1750) were transfected into HEK293 cells without or with two doses of Jarid2. The asterisk indicates a significant difference when p values are less than 0.01 from a Student's paired t test, and the error bars represent the standard error of the mean.