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Sedimentation velocity analyses of MutS and MutL variants labeled with Alexa Fluor 488
| Variant |
s20,w (frictional ratio f/f0 of the dimer) |
sexp |
|||
|---|---|---|---|---|---|
| ADP | ATP | AMPPNP | +ADP | +ADP +GT42 | |
| MutS-246C/D835R | 8.2 S (1.43) | 8.4 S (1.40) | 7.9 S | 8.9 S | |
| MutS-449C/D835R | 8.3 S (1.42) | 8.4 S (1.40) | 8.4 Sa (1.40) | 8.0 S | 8.9 S |
| MutL-297C | N.D.b | 5.8 S (1.62) | 6.1 S (1.53) | ||
a Measurement was done in 25 mm Tris (instead of HEPES) (pH 7.5), 125 mm KCl, 5 mm MgCl2, 0.01 mm EDTA, and 1 μm BSA.
b Not determined.
