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. 2011 Mar 23;286(19):17351–17358. doi: 10.1074/jbc.M111.231258

FIGURE 2.

FIGURE 2.

High agr specificity of quorum-sensing signaling inhibition mediated by AP4-24H11 in S. aureus. S. aureus strains containing a plasmid with an agr P3-YFP promoter fusion (pDB59) were used to examine the quorum-quenching activity of AP4-24H11. Reporter strains representing each of the four agr groups were constructed and incubated with AP4-24H11 in concentrations ranging from 0.2 to 9.8 × 10−5 mg/ml. As controls (Ctrl), assays containing no antibody or an isotype control antibody (0.2 mg/ml) were included. For agr inhibition controls, supernatants (sup) from MW2 containing AIP-3 or LAC containing AIP-1 were included as indicated. Using AP4-24H11, strong inhibition of agr group IV and modest inhibition of agr group I was observed, whereas minimal inhibition of agr group II and III was evident only at high AP4-24H11 doses. Error bars represent S.D. of quadruplicate samples, and each experiment was repeated.