FIGURE 3.

Increased focal adhesions and differential regulation of ECM-related genes in bgn^−/0 myofibroblasts. A and B, immunocytochemical analysis of paxillin revealed increased establishment of focal adhesions (FA) in bgn^−/0 fibroblasts. FA are pointed out by white arrows. C–F, immunoblotting and mRNA expression of paxillin and vinculin 24 h after plating in 5% FCS confirmed the results obtained by immunostaining, n = 3. G–K, mRNA expression of cell surface ECM receptor CD44, the ECM-modifying enzymes plod1 (H), plod2 (I), MMP3, and MMP13 24 h after plating in 5% FCS; n = 3 (PLOD1, 2); n = 4 (MMP3, 13). Data are presented as mean ± S.E.; *, p < 0.05.