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. Author manuscript; available in PMC: 2011 May 6.
Published in final edited form as: Nature. 2010 Sep 2;467(7311):112–116. doi: 10.1038/nature09355

Figure 1. Sgs1 and Dna2 resect DNA in a reaction dependent on yeast RPA.

Figure 1

a, Purified Dna2 (80 ng) and Sgs1 (880 ng) stained with Coomassie Brilliant Blue. b, Linear pUC19 dsDNA incubated with Sgs1 and/or Dna2, and RPA (3 μM); “SSB”: SSB substituted for RPA; “Heat”: heat-denatured dsDNA; ”annealed DNA”: partial unwinding and annealing of DNA30. c, and d, Quantification of experiments as shown in b. e, Dna2 and Sgs1 physically interact in the absence or presence of RPA (lanes 4 and 5). f, Resection by Dna2 (1 nM) is specific for yeast Sgs1 helicase; RPA is 3 μM.