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. Author manuscript; available in PMC: 2011 May 6.
Published in final edited form as: Nature. 2010 Sep 2;467(7311):112–116. doi: 10.1038/nature09355

Figure 2. Sgs1, Dna2, and RPA preferentially resect the 5'-terminated strand of a DNA break.

Figure 2

a, Assay. b, Annealing of oligonucleotide to resection products (0.5 nM Sgs1, 0.5 nM Dna2, and 3 μM RPA) using probe complementary to either top (3'-) or bottom (5'-) strand, 300 nucleotides from end. c, Quantification of DNA end resection at various distances from DSB, based on experiments as shown in b.