Figure 3. RPA promotes 5'→3' degradation by Dna2 and inhibits 3'→5' degradation.
a, Duplex DNA substrates containing either a 5'- or 3'-ssDNA flap (red asterisk indicates the 32P-label) incubated with Dna2 (15 nM) and indicated RPA. b, Illustration summarizing results from panel a and Supplementary Figs. 4 and 5 showing modulation of ssDNA nuclease activities of Dna2.
