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. Author manuscript; available in PMC: 2011 May 6.
Published in final edited form as: Nature. 2010 Sep 2;467(7311):112–116. doi: 10.1038/nature09355

Figure 4. Top3-Rmi1 and MRX complexes stimulate DNA resection by Dna2-Sgs1-RPA.

Figure 4

a, Resection kinetics: Sgs1 (0.3 nM), Dna2 (1 nM), RPA (3 μM), and Top3-Rmi1 heterodimer (10 nM). b, Top3-Rmi1-dependent stimulation of DNA resection: Sgs1 (0.5 nM), Dna2 (0.5 nM) and RPA (3 μM). c, Resection kinetics in high salt buffer (5 mM Mg2+ and 100 mM Na+): Sgs1 (7.5 nM), Dna2 (1 nM), RPA (3 μM ), and Top3-Rmi1 heterodimer (15 nM). d, Quantification of experiments as in c. e, DNA unwinding in high salt buffer; RPA (3 μM). f, Resection in high salt buffer; RPA (3 μM). g, Stimulation of resection in high salt buffer by Top3-Rmi1 (15 nM) and MRX (20 nM) using Dna2 (1 nM), RPA (3 μM), Sgs1 (0, 1.3, 1.7, 2.5, 3.8, 5.7 and 8.6 nM). h, Quantification of experiments as in g. i, Unwinding of Y-structure DNA in high salt buffer: Sgs1 (200 pM); RPA (2.25 nM); and MRX (5 nM).