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. 2011 May 6;6(5):e19771. doi: 10.1371/journal.pone.0019771

Figure 5. Mitostatin affects anchorage-independent growth of prostate cancer cells.

Figure 5

A: Data from triplicate cultures of parental PC3 and LNCaP, and clones PC3 V5, PC3 B2, PC3 M2, LNCaP V5, LNCaP B1A, LNCaP B3A, and LNCaP A3A plated in soft-agar generating colonies larger than 0.2 mm in diameter. Clones over-expressing Mitostatin showed a decreased ability to form colonies, while the Mitostatin down-regulated clone, PC3 M2, showed a higher number of colonies compared to parental cells. Colonies were counted after 21 days. * P<0.05; ** P<0.01 B: Representative colonies of LNCaP parental cells (left), LNCaP B1A (middle), and LNCaP A3A (right) are shown after 21 days (magnification ×200).