Figure 5. Lysozyme c-1 and its muramidase activity were non-detectable in An. gambiae midguts.

(A) A representative Western blot showing an absence of protein cross-reactivity with 9122 antibodies in the midguts as compared to salivary glands. (M = Protein marker). (B) Turbidometric assay showing absorbance values of different samples subtracted from control (which contained only buffer). Muramidase activity was non-detectable in the midgut tissues (n = 10) as compared to positive control (E.coli produced recombinant lysozyme c-1) as well as salivary glands (n = 10 pairs) 24 h after incubation with Micrococcus lysodeikticus cells.