Figure 3.

Method for organizing individual cells in a microfluidic channel. (a) The microfluidic channel contains barriers that trap individual, nonadherent cells. These traps reduce the effect of shear forces that cells experience in a microfluidic channel. (b) An individual HL60 cell undergoing apoptosis over the course of 134 min. The cell was exposed to staurosporine, a cytotoxic reagent, and propidium iodide, which is impermeable to membranes and therefore reports on membrane integrity. Reprinted with permission from D. Wlodkowic et al., Anal. Chem., 81, 5517 (2009). Copyright 2009American Chemical Society.