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Effect of added Vero cell DNA on detection and quantitation of the R. rickettsii rOmpA fragment, using the Rr190.547F and Rr190.701R primer pair. Serial dilutions of two independently isolated and cloned standard plasmid preparations (plasmid P1 and P2) were made in distilled water with or without Vero cell DNA to give a final assay concentration of 0.4 μg/ml. PCR conditions were described in Materials and Methods.
