TABLE 2.
Effect of DNA extraction methods on the quantitation of R. rickettsii by SQ-PCR
| Sample | No. of rOmpA copies per μl of DNA template by indicated extraction procedurea
|
||
|---|---|---|---|
| Boiling | Boiling plus Chelex | DNeasy kit | |
| Infected Vero cells | 1.81 × 104 ± 0.32 × 104b | 3.79 × 104 ± 1.75 × 104b | 6.11 × 105 ± 0.43 × 105 |
| Renografin gradient density-purified rickettsiae | 4.52 × 104 ± 0.84 × 104b | 2.96 × 105 ± 0.11 × 105b | 4.22 × 105 ± 0.35 × 105 |
a
The data are given as means±standard errors of the means for three independent preparations, each assayed in triplicate.
b
P < 0.05 compared to DNeasy extraction.