TABLE 3.
Specificity of SQ-PCR assay for different genotypes of Rickettsia
| Isolate | Ct value (mean ± SE) | No. of DNA copies per T150 flask (mean ± SE) |
|---|---|---|
| R. rickettsii Bitterroot | 16.02 ± 0.006 | 6.07 × 108 ± 0.02 × 108 |
| R. montanensis OSU 85-930 | 17.30 ± 0.42 | 2.49 × 108 ± 0.71 × 108 |
| R. rhipicephali 12T | 17.17 ± 0.15 | 2.52 × 108 ± 0.29 × 108 |
| R. parkeri HmacA | 15.96 ± 0.05 | 6.39 × 108 ± 0.24 × 108 |
| SFG isolate 364-D | 16.40 ± 0.33 | 4.82 × 108 ± 1.23 × 108 |
| R. slovaca B | 17.95 ± 0.20 | 1.41 × 108 ± 0.21 × 108 |
| R. sibirica 246 | 16.01 ± 0.39 | 6.49 × 108 ± 2.12 × 108 |
| R. africae ETH SF-2500 | 17.32 ± 0.20 | 2.27 × 108 ± 0.32 × 108 |
| Israeli tick typhus CDC | 14.87 ± 0.08 | 1.48 × 109 ± 0.09 × 109 |
| Astrakhan spotted fever A-1 | 16.97 ± 0.23 | 3.00 × 108 ± 0.57 × 108 |
| R. honei TT118 | 18.88 ± 0.34 | 7.17 × 107 ± 2.03 × 107 |
| R. akari Hartforda | >37 | <1 |
| R. australis JCa | >37 | <1 |
| R. bellii 369C42a | >37 | <1 |
| R. canadensis McKiela | >37 | <1 |
| R. canadensis CA410a | >37 | <1 |
| R. prowazekii Breinla | >37 | <1 |
| R. typhi Wilmingtona | >37 | <1 |
a
rOmpA gene is absent from these rickettsiae or has low homology to the R. rickettsii rOmpA gene at the location of the primer sequences.