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Down-regulation of different PKC isoforms in LNCaP cells after 6 and 24 hour treatment. The levels of the indicated PKC isoforms and of actin as loading control were detected by immunoblotting in total cell lysates from cells treated for 6 or 24 hours with the indicated concentrations (1–1000 nM) of PMA, bryostatin 1 or Merle 23. The data are representative of at least three independent experiments.
