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. Author manuscript; available in PMC: 2012 Jun 1.
Published in final edited form as: Biochem Pharmacol. 2011 Mar 30;81(11):1296–1308. doi: 10.1016/j.bcp.2011.03.018

Figure 7.

Figure 7

Localization of PKC delta in LNCaP cells following treatment with PMA, bryostatin 1 or Merle 23. A) Subcellular localization of endogenous PKC delta in LNCaP cells 60 min after treatment with the indicated concentrations of PMA, bryostatin 1, Merle 23 or the combination of 100 nM PMA and 10, 100, 1000 nM bryostatin 1. The immunostaining was performed as described in Materials and Methods. B) Translocation of GFP-PKC delta in LNCaP cells. LNCaP cells plated on ibidi treated dishes for better attachment were transfected to express GFP-PKC delta and 24 hours later were treated with 1000 nM PMA, bryostatin 1 or Merle 23. The translocation of GFP-PKC delta was detected by confocal microscopy in real time with images taken every 30 sec. Images of two representative cells taken at 0, 5 and 20 min are shown. The data presented in each panel are representative of four independently performed experiments.