Agarose gel staining after electrophoresis for peripheral blood leukocyte samples collected in various HIV-infected patients. A positive signal for BDV p40 RT-nested PCR was observed at the expected size (528 bp) in lane 7, whereas lanes 2 to 6 were negative. Lane 1, molecular weight marker; lane 8, negative control (water); lane 9, negative control (positive control in the absence of reverse transcriptase); lane 10, positive control in the presence of reverse transcriptase (p40 INS transcribed into RNA).