TABLE 1.
Primer or probe | Sequence | Use | Nucleotide positionsa | Gene |
---|---|---|---|---|
Primer A | 5′-TTCATACAGTAACGCCCAGC-3′ | First-round PCR | 259-278 (S) | p40 |
Primer B | 5′-GCAACTACAGGGATTGTAAGGG-3′ | First-round PCR | 829-808 (AS) | p40 |
Primer C | 5′-GCCTTGTGTTTCTATGTTTGC-3′ | Second-round PCR | 277-297 (S) | p40 |
Primer D | 5′-GCATCCATACATTCTGCGAG-3′ | Second-round PCR | 805-766 (AS) | p40 |
Probe 1 | 5′-CCGGCCATCCCATGGTGAGAC-3′ | Hybridization | 575-595 (S) | p40 |
Primer D2 | 5′-TGACCCAACCAGTAGACCA-3′ | First-round PCR | 1387-1405 (S) | p24 |
Primer A1 | 5′-GTCCCATTCATCCGTTGTC-3′ | First-round PCR | 1865-1847 (AS) | p24 |
Primer D3 | 5′-TCAGACCCAGACCAGCGAA-3′ | Second-round PCR | 1443-1461 (S) | p24 |
Primer A2 | 5′-AGCTGGGGATAAATGCGCG-3′ | Second-round PCR | 1834-1816 (AS) | p24 |
Probe 2 | 5′-TCCAGACAGCTCAGCGGTGCG-3′ | Hybridization | 1627-1647 (S) | p24 |
S, sense; AS, antisense.