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. 2003 Dec;41(12):5577–5581. doi: 10.1128/JCM.41.12.5577-5581.2003

TABLE 1.

Primers and probes used in this study

Primer or probe Sequence Use Nucleotide positionsa Gene
Primer A 5′-TTCATACAGTAACGCCCAGC-3′ First-round PCR 259-278 (S) p40
Primer B 5′-GCAACTACAGGGATTGTAAGGG-3′ First-round PCR 829-808 (AS) p40
Primer C 5′-GCCTTGTGTTTCTATGTTTGC-3′ Second-round PCR 277-297 (S) p40
Primer D 5′-GCATCCATACATTCTGCGAG-3′ Second-round PCR 805-766 (AS) p40
Probe 1 5′-CCGGCCATCCCATGGTGAGAC-3′ Hybridization 575-595 (S) p40
Primer D2 5′-TGACCCAACCAGTAGACCA-3′ First-round PCR 1387-1405 (S) p24
Primer A1 5′-GTCCCATTCATCCGTTGTC-3′ First-round PCR 1865-1847 (AS) p24
Primer D3 5′-TCAGACCCAGACCAGCGAA-3′ Second-round PCR 1443-1461 (S) p24
Primer A2 5′-AGCTGGGGATAAATGCGCG-3′ Second-round PCR 1834-1816 (AS) p24
Probe 2 5′-TCCAGACAGCTCAGCGGTGCG-3′ Hybridization 1627-1647 (S) p24
a

S, sense; AS, antisense.