Table 1.
Proportion of samples positive using manufacturers’ designations or suggested assay cutoffs
| Never pregnant (n=268)a | Ever pregnant (n=257) a | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| Assay | Method | Class I and II positive | Class I only positive | Class II only positive | Any HLA Ab positive | Class I and II positive | Class I only positive | Class II only positive | Any HLA Ab positive |
| GTI QuickStep | ELISA | 1 (0.4%) | 2 (0.7%) | 2 (0.7%) | 5 (1.9%) | 14 (5.4%) | 24 (9.3%) | 11 (4.3%) | 49 (19.1%) |
| One Lambda LATM20 | ELISA | 1 (0.4%) | 6 (2.2%) | 1 (0.4%) | 8 (3.0%) | 11 (4.3%) | 22 (8.6%) | 17 (6.6%) | 50 (19.5%) |
| 3Ti Aegis | Flow cytometry | 1 (0.4%) | 14 (5.2%) | 2 (0.7%) | 17 (6.3%) | 29 (11.3%) | 27 (10.5%) | 19 (7.4%) | 75 (29.3%) |
| Tepnel Lifescreen | Luminex | 3 (1.2%) | 9 (3.5%) | 22 (8.5%) | 34 (13.1%) | 39 (15.7%) | 30 (12.1%) | 25 (10.1%) | 94 (37.9%) |
| One Lambda LABScreen Mixedb | Luminex | 20 (7.5%) | 39 (14.6%) | 19 (7.1%) | 78 (29.1%) | 64 (19.2%) | 54 (16.2%) | 17 (5.1%) | 135 (52.5%) |
a
Samples missing HLA antibody screen data: GTI class I and II, never pregnant = 1; Tepnel class I and II, ever pregnant= 9 (high background signal was obtained); 3Ti class I and II, ever pregnant= 1 (QNS).
b
NBG ratio of 2.2 was the manufacturer’s suggested cutoffs for the organ transplantation field.