Fig. 8.

EGFRvIII expression pattern in G48-derived cell culture. a RT-PCR results carried out with two pair of primers (P1 amplicon size: 131 bp; P2 amplicon size: 90 bp), singled out G48 as EGFRvIII-positive sample; C(−) negative control; MW molecular weight marker. b Quantitative analysis demonstrated a rapid decrease of EGFRvIII mRNA level in adherent culture at passages 5 and 10, in contrast to spherical aggregates showing EGFRvIII expression at a level similar to the tumor tissue and passage 0. Real-time PCR data were presented as means ± SD; P < 0.05 was considered significant