TABLE 2.

VNTR locus-specific primers and characteristics^a

TR name	Forward primer sequence	Reverse primer sequence	Annealing temp (°C)	Tandem repeat sequence	No. of repeats		No. of alleles	Diversityb	Found in E. coli K-12	Inside ORFc
					Mimimum	Maximum
TR1	ACTGCATGATAAGCCTCAGG		57	AAATAG	4	20	12	0.89	No	No
TR2	CGCAGTTGATACCTACGG	GGAAGGAAGCTGATAGGT	53	TGGCTC	7	58	30	0.96	No	Yes
TR3	TCTTGTCAATATAGATTGG	TGATTAAGCGTGTACTGA	50	TATCTT	3	10	8	0.71	No	Yes
TR4	GGTGATGGCTTGATATTGA	GCCACACTGCGAGTATAGAG	53	TGCAAA	2	9	7	0.58	Yes	No
TR5	GTTGATTATCATGGTATGTC	GGACAACTTGTAGTACAAG	51	AAGGTG	6	21	13	0.86	No	Yes
TR6	GATGGTTCGACTAACCGTTAT	TAGCAGATGTTCGTTCCT	53	TTAAATAATCTACAGAAG	7	12	6	0.72	Yes	Yes
TR7	CGCAGTGATCATTATTAGC	TGCTGAAACTGACGACCAGT	50	GACCAC	4	9	6	0.68	Yes	Yes

^aPrimers used for the initial amplification and sequencing of the selected tandem repeats for all isolates and characteristics of each TR locus.

^bDiversity is based on Nei's marker diversity, which is 1 − Σ(allele frequency)², and on 63 or 64 unique genotypes.

^cMost of the open reading frames (ORFs) were hypothetical based on the sequence of either Sakai or EDL933 in the National Center for Biotechnology Information database (11, 19).