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. Author manuscript; available in PMC: 2011 Nov 1.
Published in final edited form as: Cancer Res. 2010 Oct 12;70(21):8487–8497. doi: 10.1158/0008-5472.CAN-10-1013

Figure 1.

Figure 1

(A) MCF10A cells expressing 6 genes WHSC1L1, BRF2, DDHD2, PROSC, ERLIN2 and ASH2L formed expanding colonies in insulin-free medium, while MCF10A cells expressing HOOK3 and control LacZ did not form colonies. (B) In vitro growth rate of the MCF10A cells that stably over express the 5 genes (ERLIN2, WHSC1L1, DDHD2, PROSC and BRF2) relative to MCF10A-LacZ control cells in insulin deficient media. Cells were seeded into 35-mm culture wells and grown in the absence of insulin-like growth factors.