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. Author manuscript; available in PMC: 2011 Nov 1.
Published in final edited form as: Cancer Res. 2010 Oct 12;70(21):8487–8497. doi: 10.1158/0008-5472.CAN-10-1013

Figure 2.

Figure 2

(A) Top: Representative pictures of MCF10A cells that stably over express DDHD2 and WHSC1L1 genes and control cell soft agar colonies. Cells were grown for 3 weeks in soft agar and stained with the vital dye p-iodonitrotetrazolium violet. Bottom: Effects of DDHD2 and WHSC1L1 on mammary acinar morphogenesis. MCF10A-DDHD2, WHSC1L1 and control cells were cultured on a bed of Matrigel as described in Materials and Methods. Representative images of structures with staining for actin with phalloidin conjugated to Alexa Fluor-568 (red), and DAPI as a marker of nuclei (blue). (B) Soft agar colony forming efficiency in MCF10A cells stably over expressing the 6 genes (WHSC1L1, BRF2, DDHD2, PROSC, ERLIN2 and ASH2L) and control cell soft agar colonies. Data represent the mean number of colonies per low power field three weeks after seeding 105 cells per well.