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(A) WHSC1L1 expression in SUM-44 cells was analyzed by semiquantitative RT-PCR and western blot after infection with non-silencing control shRNA or WHSC1L1 specific shRNA (shRNA#2 and #6). (B). The images showed the TurboGFP fluorescence of pGIPZ-WHSC1L1 shRNAs in SUM-44 cells after 3 weeks. (C) shRNA-mediated knock-down of WHSC1L1 inhibits cell growth in breast cancer cells SUM-44 and SUM-52 with WHSC1L1 amplification.
