FIGURE 4. Molecular signatures of T cell activation are maintained in cultured PBMCs from ND and T2D patients.
Comparisons of ChIP analyses showing association of acetylated histone H3 with the IL-17 (A) or RORC (B) promoters in stimulated or unstimulated PBMCs and P2Ts (see text) as indicated. Signals in the non-T cells from the P2T assays were below the level of detection (not shown). C, Comparisons of ChIP analyses of association of acetylated histone H3 to the IL-17 or RORC promoters of fresh ex vivo T cells and unstimulated or stimulated P2Ts from ND (white bars) and T2D (black bars) donors as indicated. Bars show average and SEM. n = 3–6 for each donor cohort. All comparisons were insignificantly different (p > 0.05) by Mann–Whitney U test, indicating no changes in molecular signatures postculture for 40 h.