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Disruption of actin filaments suppresses localization of MSGb5Cer at the interface. Two-cell embryos pretreated with 0, 0.2, and 2 μg/ml of CCD were immunostained with Alexa Fluor® 488-conjugated 6E2 (green). The 3D images were constructed by stacking optical slice images of the area enclosed by the square. Scale bar: 20 μm (2D), 10 μm (3D). These images shown are representative of thirty 2-cell stage embryos.
