Figure 3.
UV-irradiation inactivated virus induced activation of Akt (a) and Erk1/2 (b) at the early time. Serum-starved MDBK cells were mock infected or infected with UV-irradiated BoHV-1 and then processed for Western blotting at the time point indicated with phosphor-Akt antibody and phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) antibody. Western blotting was performed with Akt antibody (a) and β-actin antibody (b) to detect the protein loading. Each experiment was repeated three times, and representative results are shown.