Figure 5.

Effect of PI3K inhibition with Ly294002 on the virus infection of MDBK cells at the virus binding stage (a), entry and post-entry stage (b). (a) Binding of the virus to the MDBK cells pretreated with the inhibitor or mock pretreated were determined with virus titration. (b) BoHV-1 produced in MDBK cells treated with Ly294002 at the viral entry and post-entry stages were titrated. Experiments were repeated three times, and the error bars indicate the standard deviations of three independent experiments.