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Level of free and conjugated SA in nec1 and wt plants. nec1 mutant contains significantly higher level of conjugated, as well as free SA compared to parental cv. Parkland. SA content was analyzed using reverse-phase high performance liquid chromatography in leaf tissue extracts of 14 day old plants. Average values from three biological replicates are presented, each consisting of three technical replicates. Error bars represent standard deviation.
