Table 3.
Primers used in this study
| Number | Name | Sequence |
|---|---|---|
| ANG086 | 5′-BamHI +P SAV0719 | CGGGATCCGGAATAGAATATAGAATGCAATTAGAAATG |
| ANG159 | 5-KpnI-tet | GGGGTACCTTGGTTACCGTGAAGTTACCATCACGG |
| ANG317 | 3-KpnI-SAV719 | GGGGTACCCCGAGTTCGTGTTTAAATATTATTTTTTAG |
| ANG322 | 5-AvrII-YfnI-33bp | CCGCCTAGGGAACTTAAAGTGTTTAAGAAAGTAGAGGTTGCC |
| ANG323 | 3-BglII-YfnI | GAAGATCTGCAATGCGCCCGCTCAAGGCTCTTTTTCATCTTA |
| ANG326 | 5-AvrII-YflE-32bp | CCGCCTAGGGCTCGAACTGGATCGGAAAAAAGGAGTGTAACA |
| ANG327 | 3-BglII-YflE | GAAGATCTAAAGCGGAGAGGGCAACCTCTCCGCTTTTTCTTA |
| ANG328 | 5-BamHI-YqgS- with P | CGGGATCCGTCGAGAAAACATTCCGCAAATGCGCGTTTCCG |
| ANG329 | 3-KpnI-YqgS | GGGGTACCCCGCTCACTTCGATGCGGGAGACATTGTGATTA |
| ANG330 | 5-AvrII-YqgS-34bp | CCGCCTAGGCTGATTTTTTTGAGCGTGCTGCATAGGAGGTTG |
| ANG331 | 3-BglII-YqgS | GAAGATCTCCGCTCACTTCGATGCGGGAGACATTGTGATTA |
| ANG334 | 5-AvrII-YvgJ-33bp | CCGCCTAGGCAGATGATCAAGAAAACGTGAGGAGTCATATTG |
| ANG337 | 3-BeglII-YvgJ (2) | GAAGATCTGGACTACAAGGCGAATCTGTCTCATTTAAA |
| ANG579 | 5-BamHI-YflE-Cterm | CGGGATCCGATTCCAGCGACGTAACGGAAGTAG |
| ANG580 | 3-XbaI-YflE with stop | CGTCTAGAGGGCAACCTCTCCGCTTTTTCTTATTTATC |
| ANG581 | 5-BamHI-YfnI-Cterm | CGGGATCCAGCAGCGATGATTTAACAAGTGTCGAG |
| ANG582 | 3-XbaI-YfnI with stop | CGTCTAGAGCGCCCGCTCAAGGCTCTTTTTCATCTTA |
| ANG583 | 5-BamHI-YvgJ-Cterm | CGGGATCCGATGAAGACAGTATAACTGCCATTAAAAAC |
| ANG584 | 3-XbaI-YvgJ with stop | CGTCTAGACCCTCTTGATAGAGGGATTTTTTCA |
| ANG585 | 5-BamHI-YqgS-Cterm | CGGGATCCGACAGCAACAGCCTGACGGAGATTG |
| ANG586 | 3-XbaI-YqgS with stop | CGTCTAGACTTCGATGCGGGAGACATTGTGATTA |
| ANG775 | R-YqgS-PLtas | CGTTTTTCGCATGATTCTTTCCCCCGTTATTTAGATAATAAATC |
| ANG796 | N-F-PltaS-YqgS | GGGGAAAGAATCATGCGAAAAACGTTTTTTTCGAAGATTTC |
| ANG800 | 3-SalI-pCL55 | ACGCGTCGACCACGTTTCCATTTATCTGTATACGGATC |
| ANG821 | 3-P-pCL55 | P-CACGTTTCCATTTATCTGTATACGGATC |
| ANG826 | 5′AvrII-PltaS-yqgS | CCGCCTAGGCTAAATAACGGGGGAAAGAATCATG |
| ANG877 | 3-PstI-pCL55 | CGGCTGCAGCACGTTTCCATTTATCTGTATACGGATC |
| ANG1070 | 5-1kb-YfnI | CTTCCGAAAGACCCTGAAACGC |
| ANG1071 | 3-ApaI-YfnI | CCGGGGCCCGGCAACCTCTACTTTCTTAAACAC |
| ANG1072 | 5-XhoI-YfnI | CCGCTCGAGCTATCATTACGGCAAGGAGAAAGAAATC |
| ANG1073 | 3-1kb-YfnI | CCCATCTTTGGCAAGGTTCTTCAGC |
| ANG1076 | 5-1kb-YflE | GATTGTCTGTTTGAAAATGTATAAAGG |
| ANG1077 | 3-ApaI-YflE | CCGGGGCCCTGTTACACTCCTTTTTTCCGATCCAG |
| ANG1078 | 5-XhoI-YflE | CCGCTCGAGCTTCCGAAACGTCAAAGGATAACGAAG |
| ANG1079 | 3-1kb-YflE | CAACTCGTTTGGAGAGTGGATGCTC |
| ANG1082 | 5-1kb-YqgS | GCAAGCTGGATGAGCTGCAAAAACC |
| ANG1083 | 3-ApaI-YqgS | CCGGGGCCCCATTCAACCTCCTATGCAGCACGCTC |
| ANG1084 | 5-XhoI-YqgS | CCGCTCGAGCGGAAAGAAAAACAAATGCTTGATC |
| ANG1085 | 3-1kb-YqgS | CGGCTCCGTAAGCGGAGAGATTCCC |
| ANG1088 | 5-1kb-YvgJ | TAGAGCCAAGCCCTATCCGCATGTGG |
| ANG1089 | 3-ApaI-YvgJ | CCGGGGCCCCAATATGACTCCTCACGTTTTCTTG |
| ANG1090 | 5-XhoI-YvgJ | CCGCTCGAGGTGACCTGCTCAGGTTTTCCGAATG |
| ANG1091 | 3-1kb-YvgJ | CTTACCGTGTCGGAAGGCAGATGCG |
Relevant restriction sites in primer sequences are underlined.