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. Author manuscript; available in PMC: 2012 May 6.
Published in final edited form as: Cell Stem Cell. 2011 May 6;8(5):552–565. doi: 10.1016/j.stem.2011.02.021

Figure 1.

Figure 1

Hh-responding cells are localized in molecularly distinct subdomains of the telogen hair follicle. Also see Figures S1, S2.

(A) X-gal staining in adult Gli1LacZ/+ skin at day 0, 9, and 21 after depilation of telogen hair to induce regeneration of the anagen follicle. b; bulge, Scale bars=100μm.

(B, C) X-gal staining of Gli1LacZ/+ and Ptc1LacZ/+ telogen follicles showing Hh-response genes in the upper bulge, lower bulge, HG, and DP. Scale bars=100μm.

(D) Relative expression levels of Gli1 and Ptc1 mRNA assessed by RT-qPCR in wild type telogen skin treated with Hh-neutralizing antibody. Error bars=SEM. club; club hair.

(E) Immunostaining of Gli1LacZ and the progenitor cell markers indicated. Blue; DAPI. *Non-specific staining. Scale bar=100μm.