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. Author manuscript; available in PMC: 2012 May 6.
Published in final edited form as: Cell Stem Cell. 2011 May 6;8(5):552–565. doi: 10.1016/j.stem.2011.02.021

Figure 2.

Figure 2

Shh from sensory nerves signals to K15(−) hair follicle epithelium in the upper telogen bulge. Also see Figures S3, S4.

(A-C) Immunostaining in adult Shh-GIFM mice after TM induction. A) DRG. Scale bar=100μm. B) Axial and longitudinal sections through cutaneous nerves. NF; neurofilament. Scale bars=10μm. C) Hair follicle. Inset; magnification of boxed area, arrowheads; YFP(+) nerve endings on hair follicle. Scale bar=100μm.

(D) Immunostaining of Gli1LacZ/+ skin showing changes in the telogen bulge after denervation. Arrowheads; normally K15(−) upper bulge. Scale bar=100μm.

(E) X-gal staining of Gli1-GIFM telogen follicles. Upper bugle cells are not labeled in skin that is denervated then induced with TM (denervate then TM). Labeled upper bulge cells persist in skin denervated after TM induction (TM then denervate). Scale bars=100μm.