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. Author manuscript; available in PMC: 2012 Apr 1.
Published in final edited form as: Arterioscler Thromb Vasc Biol. 2011 Jan 20;31(4):883–889. doi: 10.1161/ATVBAHA.110.222430

Figure 1. HKa inhibits the colony-forming capacity of EPC colonies on collagen surfaces.

Figure 1

(A) Fluorescent microscopic image of single colony expansion of initial EPCs expressing EGFP. (B) Colony forming assay. On day 1, single cell suspension was seeded on a collagen-coated plate, and cultured in the presence of 0.1% BSA or 50 nM HKa for 14 days. Representative photomicrographs of different endothelial cell clusters (2 - 200 cells) or colonies (more than 200 cells) derived from a single EPC. Scale bar represents 50 μm. (C) The percentage of single EPC-derived colonies undergoing at least 1 cell division after 14 days of culture. Large: colonies of more than 200 cells; Small: colonies of 2-200 cells. ■, BSA, □, HKa. Data represent the results of 3 independent experiments. *P < 0.001.