TABLE 1.
Comparison of the PCR-UHG-Rif assay to culture (gold standard) for detection of M. tuberculosis directly from sputum sediments
| PCR-UHG-Rif | Culturea
|
% Predictive value
|
||
|---|---|---|---|---|
| Positiveb | Negative | Positive | Negative | |
| Positivec | 695 | 117 | 85.6 | |
| Negative | 133 | 947 | 87.7 | |
| % Sensitivity-specificity | 83.9 | 89.0 | ||
a
NALC-OH-treated sputum sediments were cultured by using L/J and MODS.
b
Culture-positive samples were positive for growth in one or both culture systems.
c
Positive specimens were positive for the 193-bp M. tuberculosis-specific band on heteroduplex gel.