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. 2011 Feb 4;144(3):364–375. doi: 10.1016/j.cell.2011.01.008

Figure S3.

Figure S3

Characterization of C. elegans SAS-6 Protein Fragments and Depletion of Endogenous HsSAS-6 Using siHsSAS-6-3′UTR, Related to Figure 2 and Figure 3

(A) ITC of the C. elegans N-N interaction. Top panel: raw data representing the response to injections of ceN at high concentration into sample buffer. Bottom panel: integrated heat change (closed squares) and associated curve fit (black solid line).

(B) CD spectrum of ceN (open circles) or ceN[I154E] (closed circles) fragments.

(C) SDS-PAGE showing final purification products for AUC of ceN and ceN[I154E] recombinant proteins.

(D) SDS-PAGE showing final purification products for AUC of ceN-CC and ceN-CC[I154E] recombinant proteins.

(E) Cells left untreated (-), transfected with LO negative control siRNA (LO) or siHsSAS-6-3′UTR (si) for 48h before Western blot analysis with HsSAS-6 antibody; tubulin served as loading control. Arrows point to endogenous HsSAS-6 or HsSAS-6-GFP proteins. Dots indicate endogenous HsSAS-6 bands, stars unspecific bands.