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. 2011 Feb 4;144(3):364–375. doi: 10.1016/j.cell.2011.01.008

Figure S6.

Figure S6

Electron Microscopy of C. reinhardtii crN-CC and crN-6HR, Related to Figure 6

(A, B) Electron micrographs of crN-CC (A) and crN-CC[F145E] (B) after glycerol spraying and rotary metal shadowing. Protein concentration was 1 mg/ml for both samples. Scale bars are indicated. Arrows in panel (A) highlight the ring oligomers only obtained with crN-CC.

(C) Electron micrographs of crN-6HR ring oligomers after glycerol spraying and rotary metal shadowing. Scale bar, 50 nm.

(D) Histogram representation of mean diameters measured from crN-6HR ring oligomers shown in (C). Note that the observed mean ring diameter and their distribution are very similar to those observed with crN-CC (Figure 6F).