TABLE 3.
Transformation frequencies using rifampin- and ciprofloxacin-resistant (single gyrA mutation) donorsa
| Strain | opa type | Phenotype | Rifampin
|
Ciprofloxacin
|
||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Donor
|
Transformants
|
Donor
|
Transformants
|
|||||||
| Mean no. of transformants/μg of donor DNA (SD) | Mean frequency of transformants (SD) | opa type | Phenotype | Mean no. of transformants/μg of donor DNA (SD) | Mean frequency of transformants (SD) | opa type | Phenotype | |||
| opa-1 strain | 1 | PAOU IB-2 | 12.62 (5.82) | 1.37 × 10−7 (2.17 × 10−6) | 1 | PAOU IB-2 | 0.25 (0.16) | 2.85 × 10−7 (4.03 × 10−7) | 1 | PAOU IB-2 |
| opa-4 strain | 4 | A IB-3 | 26.40 (2.32) | 1.03 × 10−3 (2.4 × 10−3) | 4 | A IB-3 | 10.32 (6.02) | 2.89 × 10−4 (3.94 × 10−4) | 4 | A IB-3 |
| Clinical isolate | NR IB-6 | 16.45 (5.01) | 2.12 × 10−5 (8.9 × 10−6) | NR IB-6 | 15.22 (12.42) | 2.10 × 10−4 (2.54 × 10−4) | NR IB-6 | |||
a
Values are means of results of three experiments for ciprofloxacin transformation and four experiments for rifampin transformation. Values for the opa-1 and opa-4 groups are means for two separate isolates from the clusters. Twenty rifampin- and 20 ciprofloxacin-resistant transformants of each opa group (10 per strain) from each of the separate transformation experiments were opa typed and phenotyped.