Table 3.
Back-calculated concentrations of midazolam calibration standards in human plasma
| Nominal Concentration (ng/ml) | Calculated Concentration (ng/ml)
|
Mean | SD | %CV | %Difference | |||||
|---|---|---|---|---|---|---|---|---|---|---|
| Run 1 | Run 2 | Run 3 | ||||||||
| 0.25 | 0.25 | 0.26 | 0.24 | 0.27 | 0.26 | 0.27 | 0.26 | 0.01 | 4.4 | -3.6 |
| 0.5 | 0.51 | 0.50 | 0.42 | 0.50 | 0.48 | 0.49 | 0.48 | 0.03 | 6.6 | 3.6 |
| 0.98 | 0.90 | 0.99 | 0.94 | |||||||
| 1 | 1.06 | 1.03 | 0.99 | 0.06 | 5.7 | 1.2 | ||||
| 5 | 3 | 8 | 7 | |||||||
| 2.5 | 2.59 | 2.56 | 2.41 | 2.39 | 2.58 | 2.59 | 2.52 | 0.09 | 3.7 | -0.8 |
| 5 | 5.17 | 5.36 | 5.41 | 4.94 | 5.08 | 5.04 | 5.16 | 0.18 | 3.6 | -3.3 |
| 10 | 10.7 | 10.5 | 9.45 | 9.36 | 10.2 | 10.2 | 10.1 | 0.6 | 5.4 | -0.7 |
| 25 | 23.8 | 26.3 | 21.9 | 22.4 | 25.3 | 23.9 | 23.9 | 1.7 | 7.0 | 4.3 |
| 50 | 51.8 | 54.9 | 50.1 | 44.3 | 53.9 | 53.4 | 51.4 | 3.9 | 7.5 | -2.8 |
| 100 | 95.4 | 108 | 90.8 | 102 | 102 | 109 | 101 | 7 | 7.0 | -1.2 |