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LBH589 causes acetylation of histones and non-histone proteins in adult T-cell leukemia/lymphoma (ATLL) cells. Cells were treated with either vehicle or the indicated concentrations of LBH589 for 24 h. Whole-cell lysate was prepared, and (a and c) western blotting was performed. (b) For each sample, 100 μg of cell lysate was used for immunoprecipitation with a monoclonal antibody to heat shock protein 90kDa α (HSP-90), and western blotting was performed.
