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. Author manuscript; available in PMC: 2012 Apr 18.
Published in final edited form as: Chem Res Toxicol. 2011 Mar 18;24(4):505–514. doi: 10.1021/tx100344e

Figure 1.

Figure 1

LC-MS/MS analysis of peptides released from HSA exposed to 1:1 mixture of KCN/K13C15N, following treatment with base. Upper panel is an extracted ion chromatogram from m/z 956 to 969. The peptide eluting at tR 45.94 min is the doubly charged peptide (m/z 967.4) released by incubation of purified HSA in 6 M GuHCl/0.1 M NaOH at 50°C for 3 hr and separated by LC using the gradient and MS/MS conditions cited under Experimental procedures. The lower panel shows the partial MS/MS product ion spectrum of the doubly charged peptide (m/z 967.4) from m/z 1100–1300. The major product ions observed at m/z 1130.50/1132.46, 1201.45/1203.50, and 1272.49/1274.51 are consistent with the anticipated isotope distribution of any itcCys peptides released from HSA, and the partial HSA sequence of itcCys-Phe-Ala-Glu-Glu-Gly-Lys-Lys-Leu-Valb10-Alab11-Alab12.