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. Author manuscript; available in PMC: 2012 Apr 18.
Published in final edited form as: Chem Res Toxicol. 2011 Mar 18;24(4):505–514. doi: 10.1021/tx100344e

Figure 2.

Figure 2

LC-MS/MS trace of a mixture of itcCys peptides prepared from the Cys567SCN and, Cys567S13C15N adducts in HSA and the full length-deuterium labeled (D6) cyanylated peptide. The deuterium (D6) labeled peptide was added to the HSA solubilized in 6 M GuHCl/0.1M NaOH just prior to heating at 50°C for 3 h. Upper panel is the ion current (m/z 963 to m/z 973) scan obtained by isocratic elution of the mixture and MS/MS analysis. Lower panel is a MS/MS product ion spectrum within in a restricted window of m/z 1271 to m/z 1281, showing the product b12 ion of interest in the peak eluting at 16.61 min. The uniquely high relative abundance of the 1272.72 isotope peaks at m/z 1274.64 and m/z 1278.68 confirm the presence of the peptides produced, respectively, from the itcCys567S13C15N HSA adduct and the deuterium labeled (D6) cyanylated peptide.