TABLE 1.
Sequest AA Sequence Assignment of Peptide Released from HSA by GuHCl/NaOHa
| Sequence | AA Number | b ions | y ions | AA Number |
|---|---|---|---|---|
| itcCys | 1 | 130.0 | 1932.0 | 19 |
| Phe | 2 | 277.1 | 1803.0 | 18 |
| Ala | 3 | 340.1 | 1655.9 | 17 |
| Glu | 4 | 477.2 | 1584.9 | 16 |
| Glu | 5 | 604.2 | 1455.9 | 15 |
| Gly | 6 | 663.2 | 1326.8 | 14 |
| Lys | 7 | 791.3 | 1269.8 | 13 |
| Lys | 8 | 919.4 | 1141.7 | 12 |
| Leu | 9 | 1032.5 | 1013.6 | 11 |
| Val | 10 | 1131.6 | 900.5 | 10 |
| Ala | 11 | 1202.6 | 801.4 | 9 |
| Ala | 12 | 1273.6 | 730.4 | 8 |
| Ser | 13 | 1360.7 | 659.4 | 7 |
| Gln | 14 | 1488.7 | 572.3 | 6 |
| Ala | 15 | 1559.8 | 444.3 | 5 |
| Ala | 16 | 1630.8 | 373.2 | 4 |
| Leu | 17 | 1743.9 | 302.2 | 3 |
| Gly | 18 | 1800.9 | 189.1 | 2 |
| Leu-OH | 19 | 1914.0 | 132.1 | 1 |
a
HSA not treated with K13C15N was processed and assayed using the conditions described under Experimental Procedures. Amino acid assignments were made from the full (M+2H+)2+ scan 967.4@cid 35.00 (m/z 255.00–1945) of the peptide eluting at 45.82 min. Bolded b and y ions were identified from the scan.