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. Author manuscript; available in PMC: 2011 May 10.
Published in final edited form as: J Control Release. 2010 Jan 13;143(3):290–301. doi: 10.1016/j.jconrel.2010.01.004

Fig. 7.

Fig. 7

Effect of Dox/P85 formulations on apoptosis in C57BL/6 mice with 3LL-27M solid tumors. Mice were treated with (A) saline, (B) Dox alone, (C) Dox/0.02 % P85. Twenty four hrs after the last injection, tumors were isolated, embedded in paraffin, and apoptotic DNA degradation levels were assessed by TUNEL method. (D) Apoptosis level was measured for samples in Panels A–C as the function of TUNEL positive area using ImageJ software. (E) RT-PCR data for expression of caspase 8 (white bars) and caspase 9 (black bars) in 3LL-27M solid tumors treated with Dox and Dox/P85 formulations at different P85 concentrations. Each point represents the mean ±SEM of four animals per time point. Statistical comparisons are made between the treated and the control groups injected with the same volume of the saline: * p< 0.05, ** p < 0.005.