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. Author manuscript; available in PMC: 2011 May 9.
Published in final edited form as: Biochem Pharmacol. 2009 Jul 23;78(7):889–897. doi: 10.1016/j.bcp.2009.07.010

Fig. 6. Schild analysis for mecamylamine inhibition of nicotine-evoked [3H]NE overflow from superfused rat hippocampal slices.

Fig. 6

After collection of the third basal sample, slices were superfused with buffer in the absence and presence of mecamylamine (MEC; 1 nM-10 μM; between-groups factor) for 30 min before the addition of nicotine (1 – 300 μM; within subjects factor) to the buffer, and superfusion continued for an additional 30 min. Control is the concentration-response for nicotine in the absence of mecamylamine, and the nicotine concentration response was determined contemporaneously for each concentration of mecamylamine. Concentration-response curves were generated using nonlinear regression. Curves illustrated for the 0.1 and 1 μM mecamylamine are superimposed. Data are presented as mean ± S.E.M. total [3H]NE overflow during the 30-min exposure to nicotine in the absence or presence of mecamylamine; n = 4-5 rats/mecamylamine concentration; control, n = 16 rats.