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. Author manuscript; available in PMC: 2011 Oct 15.
Published in final edited form as: J Immunol. 2010 Sep 15;185(8):4691–4697. doi: 10.4049/jimmunol.1001133

FIGURE 4.

FIGURE 4

TSP-1 expressed by APCs confers resistance to phenotypic maturation in inflammation. A, In vitro activation of TSP-1 null APCs results in enhanced frequencies of fully matured APCs. APCs were collected fresh from WT versus TSP-1 null mice (n = 3 mice per group) or subsequently activated for 72 h in vitro (TNF-α, 50 ng/ml) and evaluated via flow cytometry. B, In vitro neutralization of TSP-1 via Ab blockade similarly leads to enhanced maturation of APCs, including DCs. Activated APCs for 24 h were treated with TSP-1 neutralizing Ab (10 µg/ml) or isotype control Ab (10 µg/ml) and evaluated via flow cytometry. C, In vivo inflammation in TSP-1 null mice also leads to enhanced frequencies of fully mature APCs. Thermal cautery was administered to the corneas of WT versus TSP-1 null mice (n = 8 corneas pooled per group). Corneas were subsequently collected, digested enzymatically, and evaluated by flow cytometry. Data are representative of at least two experiments.