FIG. 3.

Deduced amino acid sequence of the NSP4 protein of Italian buffalo strain 10733 and of a selection of strains representing the five distinct NSP4 genotypes (12, 47). The potential glycosylation sites are indicated by asterisks. The highly conserved cysteine (•) and methionine (▾) residues and the putative VP4 and double-layered particle (DLP)-binding regions are shown. The enterotoxic peptide of simian rotavirus SA-11 strain at amino acids 114 to 135 and tyrosine-131 (○), considered a key residue for enterotoxigenic activity, are shown (19). Also, the hypervariable region (amino acids 135 to 141) associated with altered virulence in mouse is shown (12). Abbreviations: av, avian; bo, bovine; bu, buffalo; ca, canine; eq, equine; fe, feline; go, goat; hu, human; la, lapine; mu, murine; po, porcine; si, simian.